| Tool name | Aceprimer |
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| URL | http://elegans.bcgsc.bc.ca/aceprimer/aceprimer.shtml |
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| Important features | 1. Allows the user to design primers for C. elegansA0genome.
2. Allows the user to search for deletion alleles in Caenorhabditis elegans gene. |
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| Citations | McKay SJ, Jones SJ. AcePrimer: automation of PCR primer design based on gene structure. Bioinformatics. 2002 Nov;18(11):1538-9. PubMed PMID: 12424127. |
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| Year of publication | 2002 |
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| Rank by usage frequency | 100 |
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| Comments | |
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| Function | Real time PCR, General PCR, Primer specificity checking |
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| Category | Online, Free |
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| Features | 1. Allows the user to design poison, nested and RT PCR primers.
2. Designs PCR primers to search for deletion alleles in C.elegans gene and also design primers for nested PCR.
3. Has an option to specify the region and chromosome number to design primers.
4. Designs primers for each exons and displays the graphical view of it.
5. Allows the user to check the specificity of primer pairs against the entire C.elegans genome.
6. Displays the transcript variants of target gene graphically. |
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| Description | For amplification and expression of specific splice variants. |
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